FIG. 3.

Skp2B expression in the mammary glands reduces REA levels. (A) Four separate MMTV-Skp2B founders were used for breeding, and the mammary gland of one female of the second generation from each line was tested for Skp2B expression by quantitative RT-PCR using Skp2B-specific primers. WT, wild type. (B) The expression of Skp2B was tested by immunohistochemistry on mammary gland sections of a wild-type female (left) and a female from the Skp2B-4 transgenic line (right) by use of anti-Skp2 antibody. (C) Western analysis of the levels of REA in the mammary glands of a wild-type mouse and of three MMTV-Skp2B transgenic mice.