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. 2007 Sep 4;27(21):7631–7640. doi: 10.1128/MCB.00543-07

FIG. 7.

FIG. 7.

Tom1L1 that does not associate with CHC accumulates in caveolae. (A and B) Tom1L1 that does not associate with CHC accumulates in caveolae. (A) Tom1L1 and caveolin levels in CEF of HEK 293 cells transfected with Tom1L1 constructs and control or CHC siRNAs, when indicated. The levels of CHC, Tom1L1, and tubulin from the whole-cell lysate (WCL) are also shown. wb, Western blotting; ip, immunoprecipitation; wt, wild type; αcaveolin, anticaveolin; αTom1L1.2, anti-Tom1L1.2; αCHC, anti-CHC; αtubulin, antitubulin. (B, left panel) Statistical analysis (mean ± standard deviation [n = 3]) of the percentage of the Tom1L1 level in CEF shown in panel A. (B, right panel) Statistical analysis of Tom1L1-caveolin colocalization at the periphery of NIH 3T3 cells. Cells seeded on coverslips were transfected with the indicated Tom1L1 construct together or not with the indicated siRNA for 48 h. Cells were then fixed and processed for immunofluorescence as described in Materials and Methods. The percentage of transfected cells that exhibited Tom1L1-caveolin colocalization at the cell periphery was calculated as described in Materials and Methods. The results are expressed as the mean ± standard deviation of three independent experiments.