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Neh2 mutants that showed no observable Keap1-DC binding affinities in ITC analysis
| Neh2 construct | Modification of DLG | Modification of ETGE |
|---|---|---|
| Neh2(ΔETGE,W24D) | Trp24 to Asp | ETGE deletion |
| Neh2(ΔETGE,W24E) | Trp24 to Glu | ETGE deletion |
| Neh2(ΔETGE,D27A) | Asp27 to Ala | ETGE deletion |
| Neh2(ΔETGE,D29A) | Asp29 to Ala | ETGE deletion |
| Neh2(Δ1-33,D77A/E79A) | N-terminal deletion (amino acids 1 to 33) | Asp77 to Ala and Glu79 to Ala |
