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. 2007 Sep 4;27(21):7693–7702. doi: 10.1128/MCB.01051-07

FIG. 6.

FIG. 6.

PKA is involved in Pol III regulation, coupled to carbon metabolism. Total RNA isolated from the RAS2Val19 and cdc25-1 mutants and isogenic wild-type control cells (YPH500 and A364A, respectively), grown in glucose medium to late exponential phase (YPD medium) or transferred to glycerol medium and grown for 2 h at the permissive temperature (YPGly medium), was hybridized with intron-specific tRNALeu and U3 snoRNA probes. Bands were quantified as described in Materials and Methods. In order to determine relative levels, the amount of tRNALeu present in wild-type (wt) cells grown in YPD medium was set to 1. Lanes are numbered to facilitate explanation in the text.

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