TABLE 1.

Downregulated genes in β-catenin-knockout crypts which overlap with genes characteristic for other stem cell populations and intestinal tumors^a

Gene symbol	Gene name	Change in expression (fold)
Ankrd10	Ankyrin repeat domain 10	0.54
Apex1*	Apurinic/apyrimidinic endonuclease 1	0.44
Ascl2*	Achaete-scute complex homolog-like 2 (Drosophila)	0.40
Bcl7c	B-cell CLL/lymphoma 7C	0.64
Diap3	Diaphanous homolog 3 (Drosophila)	0.58
Gemin4*	Gem (nuclear organelle)-associated protein 4	0.53
Hat1	Histone aminotransferase 1	0.58
Hdac2	Histone deacetylase 2	0.63
Ifitm2	Interferon-induced transmembrane protein 2	0.40
Impdh2	Inosine 5′-phosphate dehydrogenase 2	0.49
Msi2	Musashi homolog 2 (Drosophila)	0.54
Narg1	NMDA receptor-regulated gene 1	0.51
Neurog3	Neurogenin 3	0.40
Notch2	Notch gene homolog 2 (Drosophila)	0.64
Rasa3	RAS p21 protein activator 3	0.64
Rgs12	Regulator of G-protein signaling 12	0.66
Rhobtb3*	Rho-related BTB domain-containing 3	0.53
Rnf138	Ring finger protein 138	0.63
Rwdd3	RWD domain-containing 3	0.65
Smarca5	SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily a, member 5	0.30
Smyd2	SET and MYND domain-containing 2	0.55
Smyd5	SET and MYND domain-containing 5	0.63
Sos2	Son of sevenless homolog 2 (Drosophila)	0.66
Sox4*	SRY-box-containing gene 4	0.41
Tiam1	T-cell lymphoma invasion and metastasis 1	0.66
Tox	Thymocyte selection-associated HMG box gene	0.52
Wdr12*	WD repeat domain 12	0.59
Zfp62	Zinc finger protein 62	0.59
Zfp148	Zinc finger protein 148	0.61
Zfp191	Zinc finger protein 191	0.42
Zfp277	Zinc finger protein 277	0.56

^aDetails for the selection can be found in the text. These represent potential intestinal stem and cancer stem cell markers. Asterisks indicate genes whose stem cell-specific expression was independently demonstrated by Van der Flier et al. (36).