FIG. 6.

Nuclear morphology showing that either inactivation of p53 or induction of hTERT renders HeLa cells sensitive to HDAP. At 48 h posttransduction with E2-expressing recombinant virus SV40/BPV-1 (MOI of 20), Sen2 cells, 16E6 cells, Sen2 and p53CTF cells, and Sen2 and hTERT cells were infected with wild-type HSV (KOS) or vBSΔ27 (Δ27) (MOI of 10). Cells infected with virus expressing E2 (+E2) and virus that did not express E2 (−E2) are shown. STS was used as a proapoptotic control. Hoechst DNA dye was added to the medium at 1 h prior to imaging to allow visualization of chromatin. Images shown were captured by fluorescence microscopy at 24 hpi with HSV-1. The percentage inside each frame is the percentage of cells containing condensed chromatin.