FIG. 7.

Biochemical assay results showing that either inactivation of p53 or induction of hTERT renders HeLa cells sensitive to HDAP. At 48 h posttransduction with E2-expressing recombinant virus SV40/BPV-1 (MOI of 20), Sen2 (A), 16E6 (B), p53CTF (C), and hTERT (D) cells were infected with wild-type HSV (KOS) or vBSΔ27 (Δ27) (MOI of 10). Cells infected with virus expressing E2 (+E2) and virus that did not express E2 (−E2) are shown. At 24 hpi with HSV, the cells were harvested, and infected-cell extracts were immunoblotted for PARP, ICP4, ICP27, gC, and TK, and p53. STS was used as a proapoptotic control. The positions of uncleaved 116,000-molecular-weight (116) and cleaved 85,000-molecular-weight (85) PARP products are indicated to the right of the immunoblots. The percentage of PARP cleavage (% Cl) was calculated using NIH Image analysis as described in Materials and Methods.