FIG. 3.

Coimmunoprecipitation of UL16 with cytoplasmic but not nuclear capsids. Vero cells were infected with HSV, and viral proteins were immunoprecipitated (IP) 18 h later from whole-cell lysates (A) or nuclear (Nuc) and cytoplasmic (Cyt) fractions (B) by using monoclonal antibodies 8F5 or 5C, which are specific for VP5 epitopes present only on mature capsids. A monoclonal antibody specific for human immunodeficiency virus (HIV) p24 was used as a negative control. The immunoprecipitated capsid and associated proteins were separated in an SDS-10% polyacrylamide gel and analyzed by immunoblotting with polyclonal rabbit serum specific for VP5 or UL16. WB, Western blotting; α, anti.