FIG. 4.

UL16-capsid interactions in virions. (A) Extracellular virions were harvested from HSV-infected Vero cells and pelleted through a 30% sucrose cushion. The samples were resuspended in TNE buffer, separated into six equal fractions, and treated as indicated for 15 min at room temperature. Following treatment, virions and capsids were pelleted through an additional sucrose cushion, resuspended in sample buffer, and separated by SDS-PAGE in 7% gels. Proteins were analyzed by immunoblotting using antibodies specific for VP5, VP16, and UL16. (B) Capsids were isolated from NP-40-treated cytoplasmic (cyt) lysates or extracellular medium by centrifugation through a 30% sucrose cushion. Capsid pellets were resuspended in sample buffer, and two different amounts were analyzed by immunoblotting using antibodies specific for VP5 and UL16. α, anti; Tryp, trypsin; Inh, inhibitors; Undil, undiluted.