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. 2007 Sep 26;81(23):12872–12880. doi: 10.1128/JVI.00974-07

FIG. 5.

FIG. 5.

Generation of Virp1-suppressed lines with the use of hairpin constructs. Virp1 mRNA levels are strongly reduced in most N. benthamiana (A) and N. tabacum (B) transgenic lines. (A) (Top) Northern blot analysis of Virp1 mRNA in N. benthamiana. Lane 1, line BX1.13 overexpressing Virp1; lane 2, wt N. benthamiana; lanes 3 to 5, transgenic N. benthamiana in which expression of Virp1 is suppressed (lines ph5.2nb, ph10nb, and ph11nb, respectively). A 5′ region of the Virp1 sequence was used as a probe to avoid detection of the hairpin transcript. (Bottom) Hybridization of the same membrane with an 18S rRNA probe as a loading control. (B) (Top) Northern blot analysis of Virp1 expression levels in transgenic N. tabacum lines. Lane 1, wt N. tabacum; lanes 2 and 3, transgenic N. tabacum in which expression of Virp1 is suppressed (lines ph10nt and ph4nt, respectively). A 5′ region of the Virp1 sequence was used as a probe to avoid detection of the hairpin transcript. (Bottom) Loading control (18S rRNA).