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. 2007 Sep 26;81(23):12872–12880. doi: 10.1128/JVI.00974-07

FIG. 6.

FIG. 6.

Transgenic N. benthamiana and N. tabacum lines suppressed for Virp1 cannot be infected by PSTVd. Detection of viroid infection by Northern blot analysis of RNA extracted from the upper leaves of transgenic lines 6 weeks p.i. Samples were separated by polyacrylamide gel electrophoresis on 6% gels. The probe was negative-strand RNA transcript of PSTVd KF 440-2 strain. (A) Lane 1, in vitro-transcribed PSTVd; lanes 2 and 3, wt N. benthamiana plants infected with 30 and 100 μl of inoculum, respectively; lanes 4 to 14, Virp1-suppressed transgenic lines, two or three plants each; lanes 4 to 6, line ph5.2nb; lanes 7 to 9, line ph10nb; lanes 10 to 12, line ph11nb; lanes 13 and 14, line ph20nb. (Bottom) The same membrane was hybridized with a U6 snRNA riboprobe as a loading control. (B) Lane 1, ph4nt; lane 2, ph10nt; lane 3, wt N. tabacum; lane 4, in vitro-transcribed PSTVd. (Bottom) Loading control (18S rRNA). C, size of circular form of viroid; L, size of linear form of viroid. The same source of inoculum was used for all experiments.