FIG. 6.

2A interaction. HeLa cells infected with vEC₉ (MOI of 10) cells were harvested at 3, 4, or 5 h p.i. into buffer containing 150 mM KCl. After a clarification step to remove nuclei, the extracts were pelleted at 200,000 × g and then washed with increasingly higher salt concentrations, as described in Materials and Methods. Equivalent samples from each subsequent supernatant or pellet were retained for fractionation by SDS-PAGE and Western analysis using antibodies against eIF4E (translation factor), 2A, or S6 (integral ribosome protein). Only the portions of the blots relative to these proteins are shown. λ, antibody target.