FIG. 6.

Infection assays with cells or HDV particles treated with AMS or TCEP prior to inoculation. HDV particles (virus) were mock treated or treated with 1:2 dilutions of 20 mM AMS or 0.5 mM TCEP for 1 h at 37°C. The drug/particle suspensions were then diluted 100-fold in fresh medium prior to inoculation to HepaRG cells. HepaRG cells (Cells) were mock treated or treated with 1:2 dilutions of 20 mM AMS or 0.5 mM TCEP for 2 h at 37°C. Cells were then washed extensively with fresh medium prior to exposure to inoculum. At day 7 postinoculation, cellular RNA was extracted for measurement of intracellular HDV RNA by Northern blot analysis. Numbers below each panel are percentages of the control value. The size (in kilobases) of HDV RNA is indicated.