TABLE 2.
Primers used in plasmid construction
| Primer | Sequence | Restriction enzyme | Construct |
|---|---|---|---|
| HC-Pro5 | 5′-TAGGATTCTGTCGAATGCCGACAATTTT-3′ | BamHI | pGBKT7-HC-Pro |
| HC-Pro3 | 5′-TACTGCAGACCAACTCTATAATGTTT-3′ | PstI | |
| PAA5 | 5′-ATGAATTCATGAGCAGAGGAAGCGGC-3′ | EcoRI | pGADT7-PAA |
| PAA3 | 5′-ATCTCGAGTCAGTCACGTTCACTAAT-3′ | XhoI | |
| PAB5 | 5′-ATGAATTCATGGGAGATAGTCAGTAC-3′ | EcoRI | pGADT7-PAB |
| PAB3 | 5′-ATCTCGAGTTACTCGACTTCAGCAAG-3′ | XhoI | |
| PAC5 | 5′-ATGAATTCATGTCTCGGAGATATGAT-3′ | EcoRI | pGADT7-PAC |
| PAC3 | 5′-ATGAGCTCTTAAGATGTTTCAGCAGC-3′ | SacI | |
| PAD5 | 5′-ATGAATTCATGGCGAGATACGATCGA-3′ | EcoRI | pGADT7-PAD |
| PAD3 | 5′-ATCTCGAGTCATGTTTCCTTCGCAGG-3′ | XhoI | |
| PAE5 | 5′-ATGAATTCATGTTTCTCACTAGAACT-3′ | EcoRI | pGADT7-PAE |
| PAE3 | 5′-ATCTCGAGTCAAAGACGAGCGATGAC-3′ | XhoI | |
| PAF5 | 5′-ATGAATTCATGTTCAGAAACCAATAC-3′ | EcoRI | pGADT7-PAF |
| PAF3 | 5′-ATCTCGAGTTACATTTCCATTGGAGC-3′ | XhoI | |
| PAG5 | 5′-ATGAATTCATGAGTAGCATTGGAACT-3′ | EcoRI | pGADT7-PAG |
| PAG3 | 5′-ATCTGCAGTTAGTCAGCATCCATCTC-3′ | XhoI | |
| PBA5 | 5′-ATGAATTCATGGATCTCAATCTCGAT-3′ | EcoRI | pGADT7-PBA |
| PBA3 | 5′-ATCTCGAGTCACATGGCCATTGGTTC-3′ | XhoI | |
| PBB5 | 5′-ATGAATTCATGTCGCAGTCTTCCGTC-3′ | EcoRI | pGADT7-PBB |
| PBB3 | 5′-ATCTGCAGTCATTCCTCCATAGCTTC-3′ | XhoI | |
| PBC5 | 5′-ATGAATTCATGTCGATCTTCGAGTAC-3′ | EcoRI | pGADT7-PBC |
| PBC3 | 5′-ATCTCGAGTCAATCCATCCTTCCCTT-3′ | XhoI | |
| PBD5 | 5′-ATGAATTCATGGAGTGTGTATTCGGT-3′ | EcoRI | pGADT7-PBD |
| PBD3 | 5′-ATCTCGAGTCAGACAACAGCAGTTGT-3′ | XhoI | |
| PBE5 | 5′-ATGAATTCATGAAGCTTGATACTAGT-3′ | EcoRI | pGADT7-PBE |
| PBE3 | 5′-ATCTGCAGTTATTCGGCTGTTGCTTC-3′ | SpeI | |
| PBF5 | 5′-ATGAATTCATGACTAAACAGCACGCG-3′ | EcoRI | pGADT7-PBF |
| PBF3 | 5′-ATCTCGAGTTAGTCTTTCCTCAGGTC-3′ | XhoI | |
| PBG5 | 5′-ATATCGATACATGACGACTTTTTCTG-3′ | ClaI | pGADT7-PBG |
| PBG3 | 5′-ATCTCGAGTTACCAGGAGCCTGCAGC-3′ | XhoI | |
| HcProBiFC5 | 5′-ATTCTAGAATGTCGAATGCCGACAATTT-3′ | XbaI | pUC-SPYCE-HC-Pro/pSPYNE-35S-HC-Pro |
| HcProBiFC3 | 5′-ATACTAGTACCAACTCTATAATGTTTTA-3′ | SpeI | |
| BIFCPAA5 | 5′-ATTCTAGAATGAGCAGAGGAAGCGGCGC-3′ | XbaI | |
| BIFCPAA3-1 | 5′-TAGGTACCGTCACGTTCACTAATGGCCG-3′ | KpnI | pUC-SPYCE-PAA/pSPYCE-35S-PAA |
| BIFCPAA3-2 | 5′-TACTCGAGGTCACGTTCACTAATGGCCG-3′ | XhoI | |
| BIFCPBB5 | 5′-ATTCTAGAATGTCGCAGTCTTCCGTC-3′ | XbaI | |
| BIFCPBB3-1 | 5′-ATGGTACCTTCCTCCATAGCTTCACC-3′ | KpnI | pUC-SPYCE-PBB/pSPYCE-35S-PBB |
| BIFCPBB3-2 | 5′-ATCCCGGGTTCCTCCATAGCTTCACC-3′ | XmaI | |
| BIFCPBE5 | 5′-ATTCTAGAATGAAGCTTGATACTAGTGG-3′ | XbaI | |
| BIFCPBE3-1 | 5′-TAGGTACCTTCGGCTGTTGCTTCCTCCA-3′ | KpnI | pUC-SPYCE-PBE/pSPYCE-35S-PBE |
| BIFCPBE3-2 | 5′-TAGGTACCTTCGGCTGTTGCTTCCTCCA-3′ | XmaI | |
| PAA1-5 | 5′-TAGAATTCATGACTGGCATGACAGCTGATTC-3′ | EcoRI | pGADT7-PAA1 |
| PAA1-3 | 5′-ATCTCGAGTCAGTCACGTTCACTAATGGCCG-3′ | XhoI | |
| PAA2-5 | 5′-ATGAATTCATGAGCAGAGGAAGCGGCGCTGG-3′ | EcoRI | pGADT7-PAA2 |
| PAA2-3 | 5′-ATCTCGAGTCAAACCATGGCAACTACTCCAA-3′ | XhoI | |
| PAA3-3 | 5′-TACTCGAGTTAGGCTAACAATCCAAGGT-3′ | XhoI | pGADT7-PAA3 |
| PBB1-5 | 5′-TAGAATTCATGTACTGTTGTGGTGCAGGA-3′ | EcoRI | pGADT7-PBB1 |
| PBB1-3 | 5′-TAGAGCTCTCATTCCTCCATAGCTTCACC-3′ | SacI | |
| PBB2-5 | 5′-TAGAATTCATGTCGCAGTCTTCCGTC-3′ | EcoRI | pGADT7-PBB2 |
| PBB2-3 | 5′-TAGAGCTCTCAGCTACCACTACCAAG-3′ | SacI | |
| PBE1-5 | 5′-TAGAATTCATGGGATATATCTCCTCACAATC-3′ | EcoRI | pGADT7-PBE1 |
| PBE1-3 | 5′-ATGAGCTCTTATTCGGCTGTTGCTTCCTCCA-3′ | SacI | |
| PBE2-5 | 5′-ATGAATTCATGAAGCTTGATACTAGTGGGTT-3′ | EcoRI | pGADT7-PBE2 |
| PBE2-3 | 5′-ATGAGCTCTTATCCAGCAATCATTGTACCAA-3′ | SacI | |
| HC-Pro1-5 | 5′-TAGAATTCATGATAGCGTTAGAGCATCTA-3′ | EcoRI | pGBKT7-HC-Pro1 |
| HC-Pro1-3 | 5′-TACTGCAGTTAACCAACTCTATAATGTTT-3′ | PstI | |
| HC-Pro2-5 | 5′-TAGAATTCATGTCGAATGCCGACAATTT-3′ | EcoRI | pGBKT7-HC-Pro2 |
| HC-Pro2-3 | 5′-TACTGCAGTTAACCATCATCAAGTGTTGT-3′ | PstI | |
| HC-Pro3-3 | 5′-TACTGCAGTTACAAGAACTTATTAACATG-3′ | PstI | pGBKT7-HC-Pro3 |