. 2007 Sep 19;81(23):12816–12826. doi: 10.1128/JVI.00432-07

TABLE 3.

Summary of MAb binding to DENV-2 DIII mutants expressed on the surfaces of yeast cells

MAb	MAb binding to mutant^a
MAb	T303Y	G304Y	K305E	K307N	K307Q	K307I	K307E	K310E	T315G	T315G and S331Y	H317Y	R323E	E327R	D329G	D329R	G330D	S331Y	E338D	T359I	E383G	P384A	P384N	N390H	N390Y
Type-specific neutralizing MAbs
1F1	<1	<1	98	100	81	36	88	48	100	51	96	100	27	37	37	2	100	100	100	3	<1	<1	100	76
3H5-1	60	1	93	86	93	43	62	41	96	88	92	100	39	99	48	100	100	84	100	5	<1	<1	100	60
6B6-10	33	2	98	80	90	67	85	44	100	<1	100	100	26	<1	2	<1	<1	100	100	100	17	31	100	76
9A3D-8	88	19	76	89	67	29	5	63	83	67	100	100	17	100	100	87	100	100	100	87	59	60	100	75
M8051122	68	1	67	92	90	67	48	17	ND	ND	ND	42	64	60	54	100	100	100	100	4	3	1	54	90
9F16	100	1	100	100	100	100	69	33	ND	ND	ND	59	83	100	80	100	89	100	100	1	2	2	78	59
9F11	89	2	100	89	100	60	60	23	ND	ND	ND	82	48	100	66	100	88	89	63	<1	1	<1	62	100
2Q1899	80.8	2	100	100	100	80	43	25	100	100	100	69	98	93	44	100	56	100	100	1	2	<1	43	100
Subcomplex-specific neutralizing MAbs
9D12	35	9	4	32	32	21	17	<1	83	76	100	100	100	45	28	72	58	74	100	74	40	3	66	32
1A1D-2	100	4	<1	<1	<1	<1	<1	<1	67	73	100	100	100	100	100	100	100	100	100	90	68	47	100	74
Nonneutralizing MAbs
5A2-7	100	40	100	100	98	62	100	27	29	5	17	100	60	66	86	100	89	78	100	38	100	83	84	89
13D4-1	74	100	94	99	59	51	100	44	100	3	2	100	67	93	87	100	93	100	8	69	100	76	90	90
WN E111	100	92	61	93	100	70	86	35	100	<1	14	61	76	17	85	100	100	74	100	100	93	74	100	86
WN E114	100	2	60	78	61	48	45	4	100	100	100	72	37	47	46	100	91	42	100	90	49	48	75	56

Values shown were obtained by dividing the total fluorescence product (percent positive population × mean linear fluorescence intensity) for a mutant for each individual antibody by the total fluorescence product for wild-type DIII. Values in bold indicate reductions in MAb binding of >80% for a given mutation. The results are averages for three to five independent experiments for each mutant and each antibody. Since the G304Y mutant had slightly less surface expression on yeast cells, the values for this mutant only were normalized to the strongest binding antibody for that mutant × 100. ND, not determined.