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. 2007 Sep 26;81(23):12936–12945. doi: 10.1128/JVI.01523-07

FIG. 1.

FIG. 1.

Enhanced rAAV transduction correlates with MRN degradation by E1b55K/E4orf6. (A) Protein degradation is required for the enhancement of rAAV transduction by E1b55K/E4orf6. WT E1b55K- or GFP-expressing HeLa cells were infected with rAAV.LacZ 24 h before superinfection with rAd.E4orf6 (MOI of 50). MG132 and epoxomicin, or dimethyl sulfoxide, were added at 2 h postinfection for another 16 h. Cells were stained or assayed for β-galactosidase. Quantitation of enzyme activity is shown in the graph below, in which error bars represent standard errors of the means for triplicate samples. inhib., inhibitors. (B) Enhanced transduction correlates with MRN degradation. Stable cell lines expressing GFP, WT E1b55K, or E1b55K mutants were infected with rAAV.LacZ in the presence or absence of rAd.E4orf6 (MOI of 50) for 24 h before β-galactosidase analysis as described for panel A. Enhanced rAAV transduction is observed only with E4orf6 in the WT E1b55K and R240A cell lines, where MRN is degraded.