VZV perturbs NF-κB binding induced by TNF-α on the targeted promoters in vivo. Mock- and VZV-infected MeWo cells were treated for 1 h with TNF-α (200 U/ml), and ChIP assays using p65, p50, or p52 antibody were carried out on total cell lysates. Real-time PCR amplification of a 100-bp fragment from the icam-1 (A) or il-8 (B) promoter encompassing the proximal NF-κB site was carried out. The results were normalized by performing PCR on the DNA input. *, statistically different (P < 0.05); **, not statistically different (P > 0.05).