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Construction of a chimeric cDNA clone of segment A of IBDV. The genomic organization of IBDV segment A is shown at the top. Open rectangles, coding sequences of segment A of strain D78; stippled rectangles, sequences of the Belgian (Belg) isolate; solid rectangles, E/Del sequences; dark shaded rectangles, GLS sequences; horizontally striped boxes, the T7 promoter. Arrowheads mark the locations of restriction enzyme sites used during cloning, and the restriction enzymes are given.
