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. 2007 Sep 19;81(23):12827–12835. doi: 10.1128/JVI.01501-07

TABLE 3.

Neutralization assay of IBDV mutants using a polyclonal serum and MAbs

Virusa Result of IIFAb with MAb:
Titer of antibodyc in neutralization assay
57 R63 67 B69 10 57 R63 67 B69 10 Anti-IBDV
rD78 − + − + + <2d >4,096 <2 4,096 2,048 4,096
PS-D78 − + + + + <2 >4,096 1,024 2,048 2,048 4,096
Mut1 − + − + − <2 >4,096 <2 1,024 <2 256
PS-Mut1 − + + + − <2 >4,096 2,048 1,024 <2 1,024
Mut2 − + − + − <2 >4,096 <2 2,048 <2 512
PS-Mut2 − + + + − <2 4,096 4096 1,024 <2 128
Mut10 + − − + − 2048 <2 <2 1,024 <2 512
Mut11 + − − + − <2 <2 <2 4,096 <2 256
D78-Belg − + + + + <2 <2 2048 4,096 2,048 256
D78-BelgS222P − + − + + <2 <2 <2 4,096 1,024 256
a

Recombinant IBDV used in the neutralization assay.

b

IIFA, indirect immunofluorescence assay.

c

Reciprocal value of the highest dilution (log2) of the antibodies in which no CPE was visible. Five MAbs (57, R63, 67, B69, 10) and one polyclonal rabbit anti-IBDV serum were used in the neutralization assay.