FIG. 6.

U2OS:vector, U2OS:100KFL, and U2OS:100KΔC cells were infected with wild type or with an 100K ts mutant adenovirus at the nonpermissive temperature (39.4°C). At 36 h p.i., cells were labeled with S³⁵ and harvested, and lysates were run on a 4 to 20% Tris-glycine gel as described in Materials and Methods. Proteins were transferred onto a polyvinylidene difluoride membrane, and the total labeled protein was visualized by using PhosphorImager. The total protein synthesis was measured by using ImageQuant and is depicted on this graph as a percentage relative to the protein synthesis in uninfected cells. The results are the average values from two independent experiments.