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UXP expressed after transfection localizes to nuclei and nucleoli. A549, A549E1 (A549 cells stably transfected with E1 genes), and 21f1 cells (HeLa cells stably transfected with DBP) were transfected with 2 μg of pcDNA3.1-UXP plasmid DNA per 35-mm well. At 48 h posttransfection cells were fixed, permeabilized, and immunostained using UXP-specific monoclonal antibodies. The secondary antibody was goat anti-mouse IgG (Alexa Fluor 488 conjugate).
