FIG. 11.

Transfected wild-type UXP acts in trans to facilitate the establishment of Ad replication centers after Ad infection with a UXP⁻ mutant. HeLa 21f1 cells (expressing DBP) or A549 cells were transfected with the pcDNA3.1-UXP plasmid. At 24 h posttransfection, cells were infected at ∼50 PFU/cell with the UXP deletion mutant VRX-006. After an additional 28 h, cells were fixed and stained for DBP and UXP. (A) Cells expressing the transfected UXP show uniform anti-nuclear DBP staining, as well as the formation of replication centers. For A549 cells on the left, the top (UXP), middle (DBP), and bottom (DAPI) panels are the same field of cells. The same is true for 21f1 cells on the right. (B) A549 cells labeled cell “a” and cell “b” from panel A are shown at larger size. Cell “a” is positive for UXP, whereas cell “b” is not.