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Comparison of DBP and UXP localization in Ad1-infected A549 cells by confocal microscopy. A549 cells were infected with Ad1 at 50 PFU/cell. At 27 h p.i., actinomycin D was added to the medium. At 30 h p.i., cells were fixed and stained for UXP (mouse monoclonal antibody; green) and DBP (rabbit polyclonal antibody; red). DBP rings are a marker for Ad replication centers. UXP staining is primarily adjacent to rather than coincident with DBP staining.
