FIG. 2.

Gating strategies used for the phenotypic analysis. p18-specific CD8⁺ T cells were identified by gating on the CD8⁺ T cells in the lymphocyte population that bind the H-2D^d/p18 tetramer. These cells were divided into effector CD62L^lo CD127^lo (E), effector-memory CD62L^lo CD127^hi (EM), and central-memory CD62L^hi CD127^hi (CM) cell subsets using gates based on the relevant “fluorescence minus one” (FMO) controls (A). We then measured the expression of the CD27 surface molecule on these E, EM, and CM cell populations (B).