FIG. 1.
A mutation in the FRNK motif of HC-Pro attenuates symptoms without reducing initial HC-Pro levels. (A) Squash (cv. Ma'ayan) leaves showing systemic symptoms at 5, 7, and 14 dpi with either severe (ZYMVFRNK) or attenuated (ZYMVFINK) ZYMV infection. Noninoculated leaves (Non-Inoc.), as a control, are shown. (B) The levels of HA-tagged HC-Pro and CP at 5 dpi were measured by Western blotting by using the antibodies indicated. A noninoculated leaf sample (NI) was loaded as a control. Arrows indicate the sizes of the nearest protein marker (M) bands. Ponceau staining was used to demonstrate equal protein loading.