FIG. 3.
Accumulations of miRNAs and miRNA*s 159, 166, and 168 in ZYMV-infected squash leaves are differently modulated over time by a mutation in the FRNK motif. RNA filter hybridization of noninoculated (H), attenuated ZYMVFINK-infected (FI), and severe ZYMVFRNK-infected (FR) squash RNA with antisense probes to the smRNAs indicated (see Fig. S1 in the supplemental material for the sequences). Ten or 20 μg of total RNA that was isolated from eight plants per treatment was loaded per lane. Days postinfection (dpi) are indicated at the top of the figure. Ethidium bromide (EtBr) staining in the tRNA zone is shown as an equal-loading control for one of the six similar gels.