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. 2007 Sep 19;81(23):12954–12961. doi: 10.1128/JVI.01601-07

FIG. 1.

FIG. 1.

NF-κB p50 and Bcl-3 immunoprecipitate on the EGFR promoter. (A) EGFR and LMP1 expression was examined by immunoblotting with C33A cells stably expressing Myc-tagged LMP1 or deletion mutant 1-231 or Δ187-351 with anti-c-myc and anti-EGFR. The bottom panel is a loading control. (B) A chromatin immunoprecipitation of p50, Bcl-3, p100/p52, p65, and RelB on the egfr promoter was performed with C33A cells stably expressing LMP1 or deletion mutant 1-231 or Δ187-351. Lanes 1 and 16 are DNA markers, lanes 2 and 17 are water PCR controls, lanes 3 and 18 are genomic DNA from C33A cells, lanes 4 to 7 are immunoprecipitated in the absence of antibody, lanes 8 to 11 are immunoprecipitated with anti-p105/p50 (α-p105/p50), lanes 12 to 15 are immunoprecipitated with anti-Bcl-3 (α-Bcl-3), lanes 19 to 22 are input DNA from all four stable cell lines, lanes 23 to 26 are immunoprecipitated with anti-p100/p52 (α-p100/p52), lanes 27 to 30 are immunoprecipitated with anti-p65 (α-p65), and lanes 31 to 34 are immunoprecipitated with anti-RelB (α-RelB).