TABLE 5.
Recovery of DSB-induced recombination events from mouse and human cell lines
| Cell line | No. of exptsa | Colony frequency (10−3)b | No. of colonies
|
||
|---|---|---|---|---|---|
| Analyzedc | Recombinantd | Nonrecombinant | |||
| Ltk−/pLB4 | 2 | 0.060 (0.058-0.062) | 25 | 20 | 5 |
| GM637/pLB4#1 | 6 | 4.34 (2.01-7.31) | 147 | 109 | 38 |
| GM637/pLB4#2 | 2 | 1.68 (1.46-1.89) | 76 | 61 | 15 |
a
Number of electroporations with pSce.
b
Average frequency of G418R colonies per electroporation. Colony frequency was calculated for each electroporation as the number of G418R colonies divided by the number of cells plated into selection. For each cell line, the range in colony frequencies for all electroporations is shown in parentheses.
c
Number of G418R colonies analyzed by PCR and AluI digestion.
d
Number of colonies analyzed that displayed the recombinant AluI restriction pattern.