FIG. 1.

Interactions between PABPC1, poly(A) nucleases, and TOB. (A) GST pull-down assays examining the interactions between PABPC1 and poly(A) nucleases. In vitro-translated and [³⁵S]methionine-labeled human CCR4, CAF1, PAN2, and PAN3 (top panel) were incubated with MagneGST particle-immobilized GST-PABPC1, and the resulting complexes were resolved by SDS-PAGE followed by Coomassie blue staining (bottom panel) for equal bait loading and then autoradiography (middle panel). PABPC1 and Paip2 were used as positive controls, whereas luciferase (Luc) was used as a negative control. (B) CCR4, CAF1, and TOB (top panel) were in vitro translated, [³⁵S]methionine labeled, and incubated in combinations with MagneGST particle-immobilized GST-PABPC1. GST pull-down (bottom panel) was performed as described for panel A. Luciferase was used as a negative control.