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. 2007 Sep 10;27(22):7991–8002. doi: 10.1128/MCB.01326-07

FIG. 8.

FIG. 8.

USF1 does not recruit RNA polymerase II to the 5′HS4 insulator at the chicken β-globin locus. A ChIP analysis of Pol II localization at the 54-kb chicken β-globin locus in 6C2 cells is shown. The IPs using anti-Pol II phosphor Ser 2 are indicated by blue bars, and those that used anti-Pol II phosphor Ser 5 are indicated by red bars. RNA polymerase levels were compared to those for a site within the 16-kb condensed chromatin region, which was assumed to be low (see Materials and Methods).