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Mapping of the Prep1 domains required for p67 interaction. Shown is the binding of in vitro-translated 35S-p67 or 35S-Pbx1b to Prep1-GST deletion mutant beads. (A) Schematic representation of Prep1 mutants and quantitation of binding of 35S-p67 (the average of three determinations). (B) Representative pull downs of 35S-p67 (left) and 35S-Pbx1b (right). (C) Representative pull downs of 35S-p160 with GST-HR1. (Top) Autoradiography. (Bottom) Coomassie staining of the gel.
