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. 2007 Sep 17;27(22):7981–7990. doi: 10.1128/MCB.01290-07

FIG. 5.

FIG. 5.

Subcellular localization of p160/p67 is modulated by ActD. (A) Confocal immunofluorescence microscopy of NIH 3T3 cells showing staining of endogenous p160 with polyclonal anti-C terminus antibodies (left). The nuclei were stained with Hoechst stain (right). The arrow points to a cell with both nucleolar and nucleoplasmic staining. (B) Confocal immunofluorescence of NIH 3T3 cells transiently transfected with a p160-FLAG construct. Shown are two cells positive for the anti-FLAG antibodies, one with nucleolar staining (arrowhead) and the other, which is dividing, with nuclear staining (arrow). (C) Confocal immunofluorescence localization of transiently transfected p67-FLAG using polyclonal anti-FLAG antibodies (left) and nuclear Hoechst staining (right). (D) Confocal immunofluorescence of endogenous p160 detected with anti-C terminus polyclonal antibodies in control (CTL)- or ActD (0.1 μM; 2 h)-stimulated NIH 3T3 cells. ActD + 24 FBS refers to cells treated with ActD as described above, washed, and left in complete medium for 24 h before microscopy. (E) Confocal immunofluorescence localization of endogenous Prep1 (red) and p160 (green) under basal conditions (left) or after ActD treatment (0.2 μM; 1 h) (right). The antibodies used are indicated. Colocalization is shown in yellow (merge).