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SBP2 cotransfection stimulates selenium labeling of Dio1 and SelP, but EFsec does not. SBP2 or EFsec expression constructs were cotransfected in HEK-293 cells with Dio1 or SelP expression plasmids. Cells were labeled with 75Se, and labeled protein in the cell lysate (dio1) or medium (selP) was analyzed by SDS-PAGE and autoradiography.
