FIG. 3.

Effects of transient knockdown of SBP2 in MSTO-211H cells on selenoprotein mRNA levels. MSTO-211H cells were transfected with SBP2 siRNAs or nontargeting siRNAs. RNA was harvested from the cells at 96 h posttransfection and used for cDNA synthesis. The levels of mRNAs for selenoproteins (A) and for selenoprotein synthesis factors, NMD factors, and antioxidant enzymes (grouped respectively) (B) relative to HPRT were analyzed by real-time PCR (n = 3). *, P ≤ 0.05, as determined by a Student's paired t test.