FIG. 4.

Effects of stable knockdown of SBP2 in MSTO-211H cells on selenoprotein mRNA levels. MSTO-211H cells were stably transfected with SBP2-targeting shRNA or nontargeting control shRNA. Aliquots of 20, 10, 5, and 2.5 μg (left to right) of total cell lysates from control and stable knockdown cells were analyzed by SDS-PAGE and Western blotting with anti-SBP2 antibody, followed by stripping and reprobing for β-actin (A). The levels of selenoprotein mRNAs (B) and of mRNAs for selenoprotein synthesis factors, NMD factors, and antioxidant enzymes (grouped respectively) (C) relative to HPRT were analyzed by real-time PCR (n = 3). *, P ≤ 0.05, as determined by a Student's paired t test.