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. 2007 Sep 10;27(22):7848–7855. doi: 10.1128/MCB.00793-07

FIG. 5.

FIG. 5.

Coimmunoprecipitation of selenoprotein mRNAs with SBP2 wild-type protein and R540Q mutant. SBP2 was immunoprecipitated from MSTO-211H cells overexpressing wild-type or mutant V5-tagged SBP2 using anti-V5 antibody. Mouse isotype control antibody was used for control immunoprecipitations (IP) of the wild-type and R540Q SBP2-overexpressing cells. RNA was extracted from the immunoprecipitates and analyzed by real-time PCR analysis to determine relative quantities of selenoprotein mRNAs. (A) Crossing points of mRNAs in SBP2 and isotype control pull-downs. (B) Changes in crossing point are given for SBP2 versus isotype control and R540Q mutant versus isotype control pull-downs. The type of SECIS element is indicated in superscript after each selenoprotein name. The asterisk indicates the noncanonical form 2 element of SelO. Amplification efficiencies per cycle are given under each selenoprotein name. n.d., not done.