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Coimmunoprecipitation of selenoprotein mRNAs with nucleolin. Nucleolin was immunoprecipitated from MSTO-211H cell lysates using antinucleolin antibody. Mouse isotype control antibody was used for control immunoprecipitations. RNA was extracted from the immunoprecipitate and analyzed by real-time PCR analysis to determine relative quantities of selenoprotein mRNAs.
