FIG. 7.

SelP SECIS 1 and SECIS 2 exhibit differential binding to SBP2 in vivo. (A) Wild-type SBP2 or mutant R540Q SBP2 was cotransfected in HEK-293 cells with SelP-lambda boxB constructs containing either the SECIS 1(S1) or SECIS 2 (S2) sequence. The recombinant SelP was pulled down with lambda boxB N-protein-linked glutathione-Sepharose. Coelution of V5-tagged SBP2 was analyzed by Western blotting. (B) SBP2 and R540Q bands were scanned and quantitated from three independent experiments. Mean densities and standard deviations are shown. (C) Blots were stripped and reprobed with anti-nucleolin antibody, and bands were scanned and quantitated.