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. 2007 Sep 17;27(22):7906–7917. doi: 10.1128/MCB.01369-07

FIG. 1.

FIG. 1.

Silencing ITSN in N1E-115 neuroblastoma cells. (A) Stable shRNAs were transfected into N1E-115 cells and either clonal (designated with a number) or polyclonal (P) cell lines were selected. ITSN expression was determined by Western blot analysis of cell lysates. The relative ITSN expression was determined by dividing the ITSN signal by the calreticulin signal and normalizing to the ratio from the N1E-115 sample. CALR, calreticulin as a normalization control for loading. (B) N1E-115-derived lines were examined for their differentiation potential by removal of serum. Similar results were obtained by differentiation with DMSO (data not shown). (C) Cell survival was quantified using a CellTiterGlo kit for measuring total ATP levels. Silencing ITSN resulted in dramatically reduced survival following serum withdraw but did not affect the growth of cells in the presence of serum (data not shown). N1E, N1E-115 cells; pSR, N1E-115 cells stably transfected with empty pSuperRetro vector.