FIG. 3.

Stable silencing of ITSN does not affect constitutive endocytosis. (A) N1E-115 cells transfected with the indicated pSR construct were incubated with biotinylated transferrin (bTfn). Uptake of transferrin was quantified by Western blot analysis with an HRP-linked streptavidin antibody. Calr, calreticulin as control for loading. (B) N1E-115 cells stably silenced for ITSN (M1635) or vector control cells (pSR) were incubated with HRP and then assayed for uptake as previously described (16).