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. 2007 Oct 1;27(23):8374–8387. doi: 10.1128/MCB.00623-07

FIG. 2.

FIG. 2.

PPAR/RXR binds to the sPLA2 promoter in VSMCs. EMSA was performed on nuclear extracts of VSMCs incubated for 24 h with IL-1β (10 ng/ml). Radiolabeled DNA probe corresponding to nucleotides −928 to −909 of the rat sPLA2 gene promoter (sPLA2-PPRE) was incubated with VSMC nuclear extracts with or without (−) excess of unlabeled probe corresponding to sPLA2, the consensus PPRE (cons), or mPPRE (lanes 1 to 4). A radiolabeled probe corresponding to the sPLA2-PPRE incubated with nuclear extracts of VSMCs transfected with vector expressing PPARα (lane 9), β (lane 10), and γ (lane 11), or with nuclear extracts of CHO transfected with vector expressing PPARα (lane 13), -β (lane 14), and -γ (lane 15) were used as a positive control for PPAR/RXR binding. Asterisks indicate nucleotides matching the consensus sequence.