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. 2007 Sep 17;27(23):8228–8242. doi: 10.1128/MCB.00374-07

FIG. 1.

FIG. 1.

Mutant p53 attenuates TGF-β1-induced cell migration and wound healing. (A) Western blot analysis depicting the protein levels of p53R175H. (B) p53-null and p53R175H-producing H1299 cells were treated with TGF-β1 and subjected to a cell migration assay. Migrated cells were fixed, stained with crystal violet, dissolved in acetic acid, and analyzed for OD by using an enzyme-linked immunosorbent assay reader. The OD values represent the amounts of migrated cells (P < 0.05). (C) p53-null and p53R175H-producing H1299 cells were seeded to confluence, scratched to create an artificial wound, and treated with TGF-β1 for 24 h. The photographs indicate wound closure. (D) Wound closure, analyzed by subtracting the distance measured between the edges of the wound at hour 24 from that at hour 0 and presented as a percentage (P < 0.001).