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. 2007 Sep 17;27(23):8228–8242. doi: 10.1128/MCB.00374-07

FIG. 6.

FIG. 6.

Mutant p53 interferes with formation of the SMAD2-SMAD4 complex. (A) p53-null and p53R175H-producing H1299 cells were treated with TGF-β1 as described in Materials and Methods. Cell lysates were immunoprecipitated with rabbit anti-SMAD2 antibody, and the precipitates were analyzed with mouse anti-SMAD4 antibodies. (B) p53R175H-producing H1299 cells were transfected with siRNA for either p53 or LacZ, treated with TGF-β1 for 30 min, and tested for the presence of p-SMAD2/3 by means of Western blot analysis. (C) p53R175H-producing H1299 cells were transfected with siRNA for p53 or LacZ, immunoprecipitated with rabbit anti-SMAD2, and analyzed for the presence of SMAD4 using a mouse anti-SMAD4 antibody. IP, immunoprecipitation; IB, immunoblotting.