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Stimulation of Rev1 activity by the Polη peptide encompassing residues 510 to 632 (Rad30-3)
| Template nucleotide | dNTP added | Enzyme | kcat (min−1) | Km (μM−1) | kcat/Km (min−1/μM−1) | Relative efficiency |
|---|---|---|---|---|---|---|
| G | dCTP | Rev1 | 1.26 ± 0.2 | 0.048 ± 0.01 | 26.3 | 1 |
| Rev1 + Rad30-3 | 3.94 ± 0.3 | 0.039 ± 0.006 | 101 | 3.84 | ||
| Apa | dCTP | Rev1 | 0.98 ± 0.05 | 1.15 ± 0.2 | 0.85 | 1 |
| Rev1 + Rad30-3 | 3.44 ± 0.3 | 0.87 ± 0.08 | 3.85 | 4.65 |
AP, abasic site.
