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. 2007 Sep 24;27(23):8065–8072. doi: 10.1128/MCB.01075-07

FIG. 3.

FIG. 3.

Functional analysis of GPR83-deficient Treg cells. (A) Proliferative responses of naïve CD4 T cells and Treg cells isolated from Gpr83−/− or control Gpr83+/− mice to the indicated concentrations of CD3 antibodies in the presence of irradiated T-cell-depleted splenocytes. The data are presented as the mean [3H]TdR incorporation for triplicate 72-h cultures ± SD. TEff, effector T cells. (B) IL-10 production by Gpr83−/− or Gpr83+/− Treg cells in response to plate-bound anti-CD3 and anti-CD28 antibody was measured by enzyme-linked immunosorbent assay kits after 24 and 48 h of culture. The data are shown as the mean IL-10 concentration for triplicate cultures ± SD. (C) In vitro analysis of suppressor capacity of GPR83-deficient Treg cells. Responder CD4 T cells and different numbers of Treg cells isolated from Gpr83−/− or control Gpr83+/− mice were cocultured for 72 h in the presence of anti-CD3 and irradiated T-cell-depleted splenocytes. The data shown as mean [3H]TdR incorporation for the triplicate cultures represent one of three identical experiments.