FIG. 6.

GPR83 is dispensable for Foxp3 up-regulation in CD25⁻ CD62L^hi CD4⁺ T cells facilitated by homeostatic proliferation or tumor growth. (A) Schematic representation of the experimental design. Foxp3 expression was examined in tumor-draining and distal LNs 14 days after the adoptive transfer of Gpr83^−/− or Gpr83^+/− CD25⁻ CD62L^hi CD4⁺ T cells into TCRβδ^−/− mice followed by the inoculation of B16.F10 melanoma cells. i.d., intradermal; sac, sacrifice. (B to E) Flow cytometric analysis of Foxp3⁺ cells within the CD4 T-cell population present in non-tumor-draining mesenteric LNs (B and C) and tumor-draining axillary and inguinal LNs (D and E). (B and D) CD4 and Foxp3 expression in LN cells gated for TCRβ⁺ cells is shown. (C and E) Each symbol represents the proportion of Foxp3⁺ T cells in a single animal. Results are from two independent experiments each with four to five animals per group.