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. 2007 Oct 8;27(23):8164–8177. doi: 10.1128/MCB.00555-07

FIG. 2.

FIG. 2.

Wnt target genes are differentially activated in neural, myogenic, and embryonic cell lines. (A) Wnt target gene activation in C17.2 neural stem-cell like cells, C2C12 myogenic cells, and E14 embryonic stem cells. Total RNA was isolated from cells treated with 25 μM (C17.2, C2C12) or 5 μM (E14) SB-216763 (lanes 1 to 3) and 200 ng/ml recombinant Wnt3a (lanes 5 to 10) as indicated. Activation of the Wnt target genes Axin2, Cdx1, and T/Bra was analyzed by RT-PCR. Amplification of GAPDH was used to control sample integrity and loading. Control reactions received no template (no templ.), cDNA samples which were mock processed in the absence of RT (−RT), or plasmid DNA or a pretested cDNA as the template (pos. control). n.a.: not analyzed. (B) Quantitative real-time PCR of Wnt target gene activation. C17.2, C2C12, and E14 cells were treated with recombinant Wnt3a for the times indicated. Activation of the Wnt target genes Axin2, Cdx1, and T/Bra is shown as induction (n-fold) compared to transcript levels in untreated cells. Data given are the average and SEM from at least three experiments.