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. 2007 Oct 1;27(23):8306–8317. doi: 10.1128/MCB.01351-07

FIG. 4.

FIG. 4.

Model of Isw2 docking to the nucleosome. (A) A summary of the mapping results for regions of Isw2 cross-linked to the nucleosomal and extranucleosomal DNA. Isw2 is schematically shown as a gray bar with conserved domains boxed in various colors. The fragments cross-linked to indicated DNA sites (17 to 18, 60 to 62, and 92 bp from the dyad axis of the nucleosome) are shown as color-coded narrow bars above the Isw2 schematic and are denoted by mapping agent, NTCB or CNBr. Beginning and ending amino acid positions for each domain and mapped fragment are given. (B) Structural model of Isw2 interacting with the nucleosomal core particle. The regions of Isw2 that interact with the SHL2 site, the entry/exit site, and extranucleosomal DNA are colored green, brown, and cyan, respectively, as in panel A and are modeled based on homology and peptide mapping data. The domains of Isw2 are labeled, and their proposed functional roles in chromatin remodeling by ISW2 are given.