TABLE 1.
A list of Isw2 markers synthesized by in vitro coupled transcription/translationa
| Marker | Fragment (residues)b | No. of amino acids | Calculated molecular mass (kDa) | Apparent molecular mass (kDa) in:
|
|
|---|---|---|---|---|---|
| Tris-glycine SDS-PAGE | BIS-Tris SDS-PAGE | ||||
| NN1 | Isw2 (1-462) | 462 | 53.7 | 57.86 | 54.10 |
| NN2 | Isw2 (1-525) | 525 | 61.0 | 63.74 | 59.27 |
| NN3 | Isw2 (1-902) | 902 | 104.5 | 108.04 | 113.71 |
| NN4 | Isw2 (1-973) | 973 | 113.1 | 116.15 | 121.49 |
| NC1 | Isw2 (464-C) | 675 | 78.7 | 81.11 | 80.35 |
| NC2 | Isw2 (534-C) | 605 | 70.4 | 71.18 | 72.34 |
| NC3 | Isw2 (903-C) | 236 | 28.0 | 32.78 | 31.80 |
| NC4 | Isw2 (974-C) | 165 | 19.4 | 25.63 | 20.79 |
| NI1 | Isw2 (464-525) | 62 | 7.2 | NA | NA |
| NI2 | Isw2 (534-902) | 369 | 42.4 | 50.01 | 47.63 |
| NI3 | Isw2 (903-973) | 71 | 8.6 | NA | 5.45 |
| CN1 | Isw2 (1-155) | 115 | 17.9 | 27.62 | 35.75 |
| CN2 | Isw2 (1-211) | 211 | 24.3 | 34.49 | NA |
| CN3 | Isw2 (1-293) | 293 | 33.9 | 44.93 | 46.82 |
| CN4 | Isw2 (1-421) | 421 | 48.9 | 53.99 | 53.77 |
| CN5 | Isw2 (1-458) | 458 | 53.2 | NA | NA |
| CN6 | Isw2 (1-492) | 492 | 57.0 | NA | NA |
| CN7 | Isw2 (1-515) | 515 | 59.7 | 62.63 | 61.00 |
| CN8 | Isw2 (1-597) | 597 | 69.2 | NA | NA |
| CN9 | Isw2 (1-668) | 668 | 77.3 | 79.39 | 79.29 |
| CN10 | Isw2 (1-897) | 897 | 103.9 | 108.63 | 106.16 |
| CN11 | Isw2 (1-911) | 911 | 105.5 | NA | NA |
| CN12 | Isw2 (1-1025) | 1025 | 119.3 | NA | 120.00 |
| CN13 | Isw2 (1-1099) | 1099 | 127.9 | NA | NA |
| CC1 | Isw2 (156-C) | 983 | 114.6 | 109.39 | 107.05 |
| CC2 | Isw2 (212-C) | 927 | 108.2 | NA | NA |
| CC3 | Isw2 (294-C) | 845 | 98.6 | 94.82 | 96.45 |
| CC4 | Isw2 (422-C) | 717 | 83.6 | 83.18 | 81.95 |
| CC5 | Isw2 (459-C) | 680 | 79.3 | 78.88 | NA |
| CC6 | Isw2 (493-C) | 646 | 75.5 | NA | NA |
| CC7 | Isw2 (516-C) | 623 | 72.7 | 70.97 | 70.70 |
| CC8 | Isw2 (598-C) | 541 | 63.3 | NA | NA |
| CC9 | Isw2 (669-C) | 470 | 55.2 | 57.18 | 57.51 |
| CC10 | Isw2 (898-C) | 241 | 28.6 | NA | NA |
| CC11 | Isw2 (912-C) | 227 | 27.0 | 30.07 | 33.47 |
| CC12 | Isw2 (1027-C) | 112 | 13.1 | 16.74 | 18.74 |
| CC13 | Isw2 (1100-C) | 39 | 4.6 | NA | NA |
| CI1 | Isw2 (156-211) | 56 | 6.4 | NA | 5.98 |
| CI2 | Isw2 (212-293) | 82 | 9.6 | NA | 23.43 |
| CI3 | Isw2 (294-421) | 128 | 15.1 | NA | 12.96 |
| CI4 | Isw2 (422-458) | 37 | 4.2 | NA | 3.82 |
| CI5 | Isw2 (459-492) | 34 | 3.9 | NA | 4.60 |
| CI6 | Isw2 (493-515) | 23 | 2.7 | NA | NA |
| CI7 | Isw2 (516-597) | 82 | 9.5 | NA | 7.68 |
| CI8 | Isw2 (598-668) | 71 | 8.1 | NA | 5.70 |
| CI9 | Isw2 (669-897) | 229 | 26.7 | NA | 32.23 |
| CI10 | Isw2 (898-911) | 14 | 1.6 | NA | NA |
| CI11 | Isw2 (912-1025) | 114 | 13.8 | NA | 25.05 |
| CI12 | Isw2 (1027-1099) | 73 | 8.5 | NA | 10.44 |
a
A marker number is given to each peptide. The first letter denotes the cutting agent (N for NTCB and C for CNBr). The second letter denotes whether the marker contains the N or C terminus of the protein; “I” indicates a marker containing neither terminus. The numbers are in order of the markers' positions relative to the full-length protein from N to C terminus. NA, not analyzed.
b
C, C terminus.