FIG. 7.

Interaction of KSRP with endogenous mRNAs. (A) HeLa cells were transfected with stKSRP or stGFP as a control for pulldown assays and with siRNA against KSRP or against GFP as a control for knockdown assays. After stimulation for 2 h with IL-1α, total RNA was isolated and subjected to microarray analysis as described in Materials and Methods. mRNAs associated with stKSRP in pulldown and increased in KSRP knockdown cells were identified. Details on mRNAs positive for both parameters (with ratios of >2 for signal intensities of stKSRP/stGFP and siRNA against KSRP/GFP) are presented in Table 1. (B) Enrichment of the indicated mRNAs in the stKSRP pulldown was confirmed by RT-PCR. (C) Input (I) and stKSRP pulldown (PD) samples from cells expressing β-globin mRNA with the indicated insertions of the KSRP 3′ UTR were analyzed for the respective mRNA by RT-PCR with β-globin mRNA-specific primers.